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Run the DHPLC Melt Program

Melt Program Descripton

Melt questions/comments

Specify single or multiple sequences:
Single sequence Multiple sequences

Enter an optional descriptive name here:


and enter the sequence(s) here (multiple sequences must be in FASTA format):

and press this:

To reset the form, press this:


Details on the algorithm, its interpretation and validation, are provided in the following publications:
  • Oefner, P.J, Underhill, P.A. (1998). DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC). Current Protocols in Human Genetics, Wiley & Sons, New York, Supplement 19, 7.10.1-7.10.12.

  • Jones, A.C., Austin, J., Hansen, N., Hoogendoorn, B., Oefner, P.J., Cheadle, J.P., O'Donovan, M.C. (1999). Optimal temperature selection for mutation detection by denaturing high performance liquid chromatography and comparison to SSCP and heteroduplex analysis. Clin. Chem., 45, 1133-1140.

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  DHPLC publication lists


  Human genes screened by DHPLC

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browse the lists online or download the complete list of human genes screened by DHPLC including references in pdf format.

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last updated 07/13/2008 | © Stanford Genome Technology Center